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sybr gold  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sybr gold
    Sybr Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sybr+gold/TRIS-BORATE-EDTA+TBE/bio_rxiv__64898__2025__12__26__696586-91-12-14
    Average 96 stars, based on 1 article reviews
    sybr gold - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms
    Article Snippet: .. Gels were washed in 1× TBE for 30 min then stained in 1× SYBR Gold (Invitrogen) in 1× TBE for 30 min. To further test for retention of AP sites in extracts, samples were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs) and eluted in 10 mM Tris–HCl (pH 8). ..

    Article Title: Synergy in viral-bacterial coinfection expedites algal bloom demise
    Article Snippet: .. After thawing, samples were stained with SYBR Gold (Invitrogen; diluted 1:10 4 in Tris-EDTA buffer) for 20 min at 80°C and analysed by flow cytometry (ex. ..

    Article Title: Random Mutagenesis for the Generation of Repertoire of Aureochrome-based Optogenetic Scaffolds
    Article Snippet: Bio-Rad Mini-PROTEAN Tetra Vertical Electrophoresis Cell and PowerPac Basic Power Supply were used for the electrophoresis, and the EMSA was performed at an ambient temperature of 4 0C. .. After the completion of the electrophoretic run, the gel was stained using SYBR Gold (Thermo Fisher Scientific) in 0.5X Tris-Borate-EDTA (TBE) buffer, by placing it on a rocking platform for 45 minutes. .. The gel was imaged using a gel documentation system (Bio-Rad).

    Article Title: OLD amputates the anticodon arm of tRNAs during P2–Lambda interference
    Article Snippet: After incubation, 6 μl of microRNA marker (NEB) was spiked into the ladder mixture. .. 3 μl of ladder and 4 μl of reaction were run on a 15 well 15% TBE–Urea gel at 180 V for 45 min. Gels were stained with SYBR gold (Thermo) in 1× TBE buffer before imaging using an Amersham Typhoon in fluorescence mode with the Cy2 filter. ..

    Article Title:
    Article Snippet: .. Gels were stained for ~5 min. with SYBR Gold (Thermo Fisher) diluted 1:10000 from the manufacturersupplied dimethylsulfoxide stock solution into deionized water. .. Fragmented and intact DNA species were observed by measuring the SYBR Gold fluorescence signal on a ChemiDoc MP imaging system (BioRad).

    Article Title: Two microbiome metabolites compete for tRNA modification to impact mammalian cell proliferation and translation quality control.
    Article Snippet: Wen Zhang , Kuldeep Lahry , Denis Cipurko, Sihao Huang, Olivia Zbihley , Amanda M. Sevilleja, Dominika Rudzka, Luke R. Frietze , Mahdi Assari , Christopher D. Katanski, Marisha Singh, Aurore Attina, Hélène Guillorit, Christopher P. Watkins, Delphine Gourlain , Didier Varlet, Jennifer Falconi, Alexandre Djiane , Christophe Hirtz , Hankui Chen, Françoise Macari, Katherine Johnson, Nicolas Chevrier , Alexandre David & Tao Pan

    Article Title: SnoBIRD: a tool to identify C/D box snoRNAs and refine their annotation across all eukaryotes
    Article Snippet: The RNA was separated on a 10% denaturing urea gel [8 M urea, 10% acrylamide:bisacrylamide (19:1), 1× TBE at pH 8.3] at 100 V in a Mini Trans-Blot Cell (Bio-Rad 153BR) until the bromophenol dye reached the bottom of the gel. .. Gels were stained with SYBR gold (Thermo Fisher Scientific S-11494) in 1× TBE buffer (1:5000). ..

    Article Title: Structurally related phage helicases trigger type III-A3 retron-mediated anti-phage defense across diverse tailed phage families
    Article Snippet: Reactions were heated at 95°C for 150 s, placed on ice, and run on a precast 10% acrylamide TBE-Urea gel (Invitrogen) at 400 V in 1× TBE running buffer preheated to ∼50°C. .. The gel was stained with SYBR Gold (Invitrogen) in 1× TBE for 15 min and visualized with ChemiDoc (Bio-Rad). ..

    Purification:

    Article Title: Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms
    Article Snippet: .. Gels were washed in 1× TBE for 30 min then stained in 1× SYBR Gold (Invitrogen) in 1× TBE for 30 min. To further test for retention of AP sites in extracts, samples were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs) and eluted in 10 mM Tris–HCl (pH 8). ..

    Polymerase Chain Reaction:

    Article Title: Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms
    Article Snippet: .. Gels were washed in 1× TBE for 30 min then stained in 1× SYBR Gold (Invitrogen) in 1× TBE for 30 min. To further test for retention of AP sites in extracts, samples were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs) and eluted in 10 mM Tris–HCl (pH 8). ..

    Flow Cytometry:

    Article Title: Synergy in viral-bacterial coinfection expedites algal bloom demise
    Article Snippet: .. After thawing, samples were stained with SYBR Gold (Invitrogen; diluted 1:10 4 in Tris-EDTA buffer) for 20 min at 80°C and analysed by flow cytometry (ex. ..

    Imaging:

    Article Title: OLD amputates the anticodon arm of tRNAs during P2–Lambda interference
    Article Snippet: After incubation, 6 μl of microRNA marker (NEB) was spiked into the ladder mixture. .. 3 μl of ladder and 4 μl of reaction were run on a 15 well 15% TBE–Urea gel at 180 V for 45 min. Gels were stained with SYBR gold (Thermo) in 1× TBE buffer before imaging using an Amersham Typhoon in fluorescence mode with the Cy2 filter. ..

    Fluorescence:

    Article Title: OLD amputates the anticodon arm of tRNAs during P2–Lambda interference
    Article Snippet: After incubation, 6 μl of microRNA marker (NEB) was spiked into the ladder mixture. .. 3 μl of ladder and 4 μl of reaction were run on a 15 well 15% TBE–Urea gel at 180 V for 45 min. Gels were stained with SYBR gold (Thermo) in 1× TBE buffer before imaging using an Amersham Typhoon in fluorescence mode with the Cy2 filter. ..



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